Recombinant Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

Function

Specifically hydrolyzes both 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) to the related monophosphates, thereby cleaning up the nucleotide pools and preventing misincorporation of 8-oxoGua into DNA and RNA (PubMed:1309939, PubMed:15850400, PubMed:9311918). It prevents replicational errors by removing an oxidatively damaged form of guanine (8-oxo-dGTP) from DNA and the nucleotide pool (PubMed:1309939). 8-oxo-dGTP can be inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions (PubMed:1309939). MutT may also ensure transcriptional fidelity, removing 8-oxo-GTP from the ribonucleotide triphosphate pool (PubMed:9311918). However, due to the lower efficiency of RNA polymerase 8-oxo-GTP incorporation, MutT is probably not a major contributor to transcriptional fidelity (PubMed:25294823). It also hydrolyzes 8-oxo-dGDP and 8-oxo-GDP to their monophosphate form (PubMed:15850400). In vitro, can also use dGTP, dGDP and other various nucleoside di- and triphosphates, with much lower efficiency (PubMed:1309939, PubMed:15850400, PubMed:1851162, PubMed:23481913). Works cooperatively with MutM and MutY to prevent accumulation in the DNA of oxidized guanine residues (PubMed:7739614)

Subunit

Monomer

• Akiyama M. et al. Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion. — Mol. Gen. Genet. (1987)
• Yura T. et al. Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. — Nucleic Acids Res. (1992)
• Blattner F.R. et al. The complete genome sequence of Escherichia coli K-12. — Science (1997)
• Hayashi K. et al. Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. — Mol. Syst. Biol. (2006)
• Schmidt M. et al. Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli. — J. Bacteriol. (1988)
• Fujita N. et al. Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. — Nucleic Acids Res. (1994)
• Bhatnagar S.K. et al. Studies on the mutator gene, mutT of Escherichia coli. Molecular cloning of the gene, purification of the gene product, and identification of a novel nucleoside triphosphatase. — J. Biol. Chem. (1988)
• Bhatnagar S.K. et al. Characterization of the mutT nucleoside triphosphatase of Escherichia coli. — J. Biol. Chem. (1991)