Recombinant Escherichia coli Beta-lactamase (ampC) — Class C beta-lactamase which confers resistance to penicillins and cephalosporins. Purity >85%.
| Product Sku | BCRECP-002080 |
| Product Description | Recombinant Escherichia coli Beta-lactamase (ampC) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 20-377aa. [Accession | P00811]. |
| Uniprot No. | P00811 |
| Gene Names | ampC |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 20-377aa |
| Species | Escherichia coli (strain K12) |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Molecular weight | 52.5kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Microbiology |
Class C beta-lactamase which confers resistance to penicillins and cephalosporins. Has benzylpenicillin- and cephaloridine-hydrolyzing activity.
Recombinant Escherichia coli Beta-lactamase (ampC) — Class C beta-lactamase which confers resistance to penicillins and cephalosporins. Purity >85%. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Class C beta-lactamase which confers resistance to penicillins and cephalosporins (PubMed:12323371, PubMed:17956081, PubMed:23043117, PubMed:33199391, PubMed:6998377). Has benzylpenicillin- and cephaloridine-hydrolyzing activity (PubMed:3264154, PubMed:3264155, PubMed:6998377). Has weak cefuroxime, cefotaxime, cefoxitin and oxacillin-hydrolyzing activities (PubMed:19913034, PubMed:3264154, PubMed:3264155)
Periplasm
Monomer
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
