Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 56.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA)

Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA) — Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. Purity >90%.

SKU: BCRECP-002806 Category:
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Product Specifications

Product SkuBCRECP-002806
Product DescriptionRecombinant Escherichia coli Glutamate decarboxylase alpha (gadA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-466aa. [Accession | P69908].
Uniprot No.P69908
Gene NamesgadA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-466aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight56.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.

Product Specifications

Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-466aa, with N-terminal 6xHis-tagged tag, molecular weight 56.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria

Subunit

Homohexamer

Gene: gadA  |  Organism: Escherichia coli (strain K12)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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