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Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated

Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated — Hydrogen peroxide (H2O2) sensor. Purity >90%.

Product Specifications

Product SkuBCRECP-003987
Product DescriptionRecombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 1-305aa. [Accession | P0ACQ4].
Uniprot No.P0ACQ4
Gene NamesoxyR
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-305aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight82.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Hydrogen peroxide (H2O2) sensor. Activates the expression of a regulon of H2O2-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA in response to H2O2. Represses transcription of phage Mu mom gene in a methylation-sensitive manner; MomR binds to the mom promoter when it is unmethylated but not if it is fully methylated.

Product Specifications

Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-305aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 82.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Hydrogen peroxide (H2O2) sensor. Activates the expression of a regulon of H2O2-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA in response to H2O2. Represses transcription of phage Mu mom gene in a methylation-sensitive manner; MomR binds to the mom promoter when it is unmethylated but not if it is fully methylated (PubMed:2551682). Binds DNA in the upstream regions of its target genes; more than one site may be present per gene (PubMed:1864839, PubMed:2471187, PubMed:2551682). OxyR is inactivated by reduction of its essential disulfide bond by glutaredoxin (Grx1, grxA), itself positively regulated by OxyR (PubMed:9497290). Also has a positive regulatory effect on the production of surface proteins that control the colony morphology and auto-aggregation ability (PubMed:15659678)

Subunit

Homodimer and homotetramer

Gene: oxyR  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Hydrogen peroxide-inducible genes activator; Morphology and auto-aggregation control protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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