Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB), Escherichia coli O45:K1 (strain S88 / ExPEC), Yeast, C-terminal 6xHis-tagged, 35.9kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB)

Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) — recombinant protein from Escherichia coli O45:K1 (strain S88 / ExPEC), expressed in Yeast. Purity >90%.

SKU: BCRECP-004225 Categories: ,
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Product Specifications

Product SkuBCRECP-004225
Product DescriptionRecombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 1-311aa. [Accession | B7MLQ3].
Uniprot No.B7MLQ3
Gene NamespyrB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-311aa
SpeciesEscherichia coli O45:K1 (strain S88 / ExPEC)
Tag InfoC-terminal 6xHis-tagged
Molecular weight35.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.

Biological Context

Pathway: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3

Product Specifications

Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) is a recombinant protein from Escherichia coli O45:K1 (strain S88 / ExPEC), expressed in Yeast, covering amino acids 1-311aa, with C-terminal 6xHis-tagged tag, molecular weight 35.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway

Subunit

Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2)

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3

Gene: pyrB  |  Organism: Escherichia coli O45:K1 (strain S88 / ExPEC)  |  Synonyms: Aspartate transcarbamylase

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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