Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB), Escherichia coli O45:K1 (strain S88 / ExPEC), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 41.9kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB)

Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) — recombinant protein from Escherichia coli O45:K1 (strain S88 / ExPEC), expressed in E.coli. Purity >90%.

SKU: BCRECP-004224 Categories: ,
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Product Specifications

Product SkuBCRECP-004224
Product DescriptionRecombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-311aa. [Accession | B7MLQ3].
Uniprot No.B7MLQ3
Gene NamespyrB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-311aa
SpeciesEscherichia coli O45:K1 (strain S88 / ExPEC)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight41.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.

Biological Context

Pathway: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3

Product Specifications

Recombinant Escherichia coli O45:K1 Aspartate carbamoyltransferase (pyrB) is a recombinant protein from Escherichia coli O45:K1 (strain S88 / ExPEC), expressed in E.coli, covering amino acids 1-311aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 41.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway

Subunit

Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2)

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3

Gene: pyrB  |  Organism: Escherichia coli O45:K1 (strain S88 / ExPEC)  |  Synonyms: Aspartate transcarbamylase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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