Recombinant Escherichia coli Probable ABC transporter periplasmic-binding protein SapA (sapA), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 66.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Probable ABC transporter periplasmic-binding protein SapA (sapA)

Recombinant Escherichia coli Probable ABC transporter periplasmic-binding protein SapA (sapA) — Not part of a putrescine export system. Purity >85%.

SKU: BCRECP-004374 Category:
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Product Specifications

Product SkuBCRECP-004374
Product DescriptionRecombinant Escherichia coli Probable ABC transporter periplasmic-binding protein SapA (sapA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 22-547aa. [Accession | Q47622].
Uniprot No.Q47622
Gene NamessapA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-547aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight66.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Not part of a putrescine export system. Very similar to a S.typhimurium protein implicated in antimicrobial peptide resistance, but the SapBCDF operon in E.coli is implicated in putrescine export.

Biological Context

Subcellular Location: Periplasm

Product Specifications

Recombinant Escherichia coli Probable ABC transporter periplasmic-binding protein SapA (sapA) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 22-547aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 66.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Not part of a putrescine export system (PubMed:27803167). Very similar to a S.typhimurium protein implicated in antimicrobial peptide resistance, but the SapBCDF operon in E.coli is implicated in putrescine export (PubMed:27803167)

Subcellular Location

Periplasm

Gene: sapA  |  Organism: Escherichia coli (strain K12)

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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