Recombinant Escherichia coli Probable phosphatidylethanolamine transferase Mcr-1 (mcr1) Protein, Escherichia coli, in vitro E.coli expression system, C-terminal 10xHis-tagged, 63.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Probable phosphatidylethanolamine transferase Mcr-1 (mcr1) Protein

Recombinant Escherichia coli Probable phosphatidylethanolamine transferase Mcr-1 (mcr1) Protein — Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Purity >90%.

SKU: BCRECP-00494TM Categories: ,
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Product Specifications

Uniprot No.A0A0R6L508
Gene Namesmcr1
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-541aa
SpeciesEscherichia coli
Tag InfoC-terminal 10xHis-tagged
Molecular weight63.1kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A confers polymyxin resistance. Confers resistance to polymyxin-type antibiotics such as colistin; in the E.coli strain W3110.

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Probable phosphatidylethanolamine transferase Mcr-1 (mcr1) Protein is a recombinant protein from Escherichia coli, expressed in in vitro E.coli expression system, covering amino acids 1-541aa, with C-terminal 10xHis-tagged tag, molecular weight 63.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A (PubMed:26603172, PubMed:29079699). Phosphoethanolamine modification of lipid A confers polymyxin resistance (PubMed:26603172). Confers resistance to polymyxin-type antibiotics such as colistin; in the E.coli strain W3110 (PubMed:26603172)

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Monomer

Gene: mcr1  |  Organism: Escherichia coli  |  Synonyms: Polymyxin resistance protein MCR-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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