Recombinant Escherichia coli Trifunctional NAD biosynthesis/regulator protein NadR (nadR), Escherichia coli (strain K12), E.coli, C-terminal 6xHis-tagged, 54.4kDa, purity >95% as determined by SDS-PAGE.

Recombinant Escherichia coli Trifunctional NAD biosynthesis/regulator protein NadR (nadR)

Recombinant Escherichia coli Trifunctional NAD biosynthesis/regulator protein NadR (nadR) — This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. Purity >95%.

SKU: BCRECP-003841 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003841
Product DescriptionRecombinant Escherichia coli Trifunctional NAD biosynthesis/regulator protein NadR (nadR) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-410aa. [Accession | P27278].
Uniprot No.P27278
Gene NamesnadR
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-410aa
SpeciesEscherichia coli (strain K12)
Tag InfoC-terminal 6xHis-tagged
Molecular weight54.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein); Cytoplasm
Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1

Product Specifications

Recombinant Escherichia coli Trifunctional NAD biosynthesis/regulator protein NadR (nadR) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-410aa, with C-terminal 6xHis-tagged tag, molecular weight 54.4kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity catalyzes the transfer of the AMP moiety of ATP to nicotinamide ribonucleotide to form NAD(+). The NMN adenylyltransferase domain also functions as the NAD and ATP sensor

Subcellular Location

Cell membrane (Peripheral membrane protein); Cytoplasm

Subunit

Homotetramer

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1

Gene: nadR  |  Organism: Escherichia coli (strain K12)

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top