Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5), Escherichia phage T7 (Bacteriophage T7), Yeast, Tag-Free, 27.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5)

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) — Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks. Purity >90%.

SKU: BCRECP-001957 Category:
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Product Specifications

Product SkuBCRECP-001957
Product DescriptionRecombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) Protein is expressed from Yeast with Tag-Free. It contains 1-232aa. [Accession | P03696].
Uniprot No.P03696
Gene Names2.5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-232aa
SpeciesEscherichia phage T7 (Bacteriophage T7)
Tag InfoTag-Free
Molecular weight27.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks. Coats the lagging-strand ssDNA as the replication fork advances and stimulates the activities of viral DNA polymerase and primase/helicase. Coordinates simultaneous synthesis of leading- and lagging-strands.

Product Specifications

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) is a recombinant protein from Escherichia phage T7 (Bacteriophage T7), expressed in Yeast, covering amino acids 1-232aa, with Tag-Free tag, molecular weight 27.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks (PubMed:11222583, PubMed:16807232, PubMed:8617248, PubMed:9079662). Coats the lagging-strand ssDNA as the replication fork advances and stimulates the activities of viral DNA polymerase and primase/helicase (PubMed:8617248). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:9651583). Together with DNA primase/helicase, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:8617248). Also promotes the formation of joint molecules (PubMed:8617248, PubMed:9079662). Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation (PubMed:1634538)

Subunit

Homodimer (PubMed:11481454, PubMed:1634538). Interacts (via C-terminus) with the viral DNA polymerase (PubMed:12766155, PubMed:15795374, PubMed:1634539, PubMed:8106511). Interacts with the viral helicase/primase (PubMed:1634539). Part of the replicase complex that includes the DNA polymerase, host thioredoxin, the primase/helicase and the single-stranded DNA binding protein (PubMed:22977246)

Organism: Escherichia phage T7  |  Synonyms: 2.5 protein; Gene product 2.5
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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