| Product SKU | BCACP-000349 |
| Product Description | Recombinant Finegoldia magna ATCC 53516 LPXTG-motif cell wall anchor domain protein, Biotinylated protein is expressed from E.coli with N-terminal 10xHis-Avi-tagged. It contains 106-470aa. [Accession | D6S9W1]. |
| Uniprot No. | D6S9W1 |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | E.coli |
| Expression Region | 106-470aa |
| Species | Finegoldia magna ATCC 53516 |
| Tag Info | N-terminal 10xHis-Avi-tagged |
| Molecular weight | 44.2 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TSHR at 10 μg/mL can bind Anti-TSHR recombinant antibody . The EC50 is 30.81-36.12 ng/mL.The VLPs is negative control. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
