Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated, Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 87.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated

Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated — Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. Purity >85%.

SKU: BCRECP-003989 Categories: , , , ,
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Product Specifications

Product SkuBCRECP-003989
Product DescriptionRecombinant Hepatitis B virus genotype D subtype ayw Protein P (P) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 334-681aa. [Accession | Q67878].
Uniprot No.Q67878
Gene NamesP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region334-681aa
SpeciesHepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight87.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid.

Product Specifications

Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated is a recombinant protein from Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D), expressed in E.coli, covering amino acids 334-681aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 87.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid. Initiation of reverse-transcription occurs first by binding the epsilon loop on the pgRNA genome, and is initiated by protein priming, thereby the 5'-end of (-)DNA is covalently linked to P protein. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA). The activity of P protein does not seem to be necessary for cccDNA generation, and is presumably released from (+)DNA by host nuclear DNA repair machinery

Gene: P  |  Organism: Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992)

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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