Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P), Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 34.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P)

Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) — Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Purity >85%.

SKU: BCRECP-003988 Categories: ,
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Product Specifications

Product SkuBCRECP-003988
Product DescriptionRecombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-265aa. [Accession | P04879].
Uniprot No.P04879
Gene NamesP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-265aa
SpeciesVesicular stomatitis Indiana virus (strain Glasgow) (VSIV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight34.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. Acts as a chaperone for newly synthesized free N protein, so-called N(0).

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) is a recombinant protein from Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV), expressed in E.coli, covering amino acids 1-265aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 34.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. Acts as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly

Subcellular Location

Virion; Host cytoplasm

Subunit

Homodimer (By similarity). Interacts with the L polymerase; the association of P and L forms the polymerase complex and positions P optimally for encapsidation of newly synthesized genomes with the nucleoprotein. Interacts (via N-terminus) with N(0). Interacts (via C-terminus) with N in ribonucleocapsid (via C-terminus); this interaction allows to package the L polymerase in the virion and positions the polymerase on the template, since P acts as a bridge between N and L (By similarity)

Gene: P  |  Organism: Vesicular stomatitis Indiana virus (strain Glasgow)  |  Synonyms: Protein M1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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