Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P), Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 34.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P)

Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) — Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Purity >85%.

SKU: BCRECP-003988 Categories: ,
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Product Specifications

Product SkuBCRECP-003988
Product DescriptionRecombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-265aa. [Accession | P04879].
Uniprot No.P04879
Gene NamesP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-265aa
SpeciesVesicular stomatitis Indiana virus (strain Glasgow) (VSIV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight34.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. Acts as a chaperone for newly synthesized free N protein, so-called N(0).

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) is a recombinant protein from Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV), expressed in E.coli, covering amino acids 1-265aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 34.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. Acts as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly

Subcellular Location

Virion; Host cytoplasm

Subunit

Homodimer (By similarity). Interacts with the L polymerase; the association of P and L forms the polymerase complex and positions P optimally for encapsidation of newly synthesized genomes with the nucleoprotein. Interacts (via N-terminus) with N(0). Interacts (via C-terminus) with N in ribonucleocapsid (via C-terminus); this interaction allows to package the L polymerase in the virion and positions the polymerase on the template, since P acts as a bridge between N and L (By similarity)

Gene: P  |  Organism: Vesicular stomatitis Indiana virus (strain Glasgow)  |  Synonyms: Protein M1

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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