Recombinant Horse Interleukin-1 beta protein (IL1B), Equus caballus (Horse), E.Coli, Tag-Free, 17.3 kDa, purity >>95% as determined by SDS-PAGE.
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Recombinant Horse Interleukin-1 beta protein (IL1B)

Recombinant Horse Interleukin-1 beta protein (IL1B) — Potent pro-inflammatory cytokine. Purity >95%.

SKU: BCACP-000728 Categories: ,
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Product SKUBCACP-000728
Product DescriptionRecombinant Horse Interleukin-1 beta protein (IL1B) protein is expressed from E.Coli with Tag-Free. It contains 116-268aa. [Accession | Q28386].
Uniprot No.Q28386
Gene NamesIL1B
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region116-268aa
SpeciesEquus caballus (Horse)
Tag InfoTag-Free
Molecular weight17.3 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIL1BInterleukin-1 beta; IL-1 beta

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

Product Specifications

Recombinant Horse Interleukin-1 beta protein (IL1B) is a recombinant protein from Equus caballus (Horse), expressed in E.Coli, covering amino acids 116-268aa, with Tag-Free tag, molecular weight 17.3 kDa, purity >95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore

Subcellular Location

Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

Subunit

Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all. Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling. Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form. Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC. Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC

Gene: IL1B  |  Organism: Equus caballus

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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