Recombinant Human adenovirus C serotype 5 Hexon protein (L3) (T273A), partial, Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human adenovirus C serotype 5 Hexon protein (L3) (T273A), partial

Recombinant Human adenovirus C serotype 5 Hexon protein (L3) (T273A), partial — Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Purity >85%.

SKU: BCRECP-003233 Categories: ,
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Product Specifications

Product SkuBCRECP-003233
Product DescriptionRecombinant Human adenovirus C serotype 5 Hexon protein (L3) (T273A) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 133-324aa(T273A). [Accession | P04133].
Uniprot No.P04133
Gene NamesL3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region133-324aa(T273A)
SpeciesHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid.

Biological Context

Subcellular Location: Virion; Host nucleus

Product Specifications

Recombinant Human adenovirus C serotype 5 Hexon protein (L3) (T273A), partial is a recombinant protein from Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), expressed in E.coli, covering amino acids 133-324aa(T273A), with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus

Subcellular Location

Virion; Host nucleus

Subunit

Homotrimer. Interacts with the capsid vertex protein; this interaction binds the peripentonal hexons to the neighboring penton base. Interacts with the hexon-linking protein VIII; this interaction tethers the hexons surrounding the penton to those situated in the central plate of the facet (PubMed:28978703, PubMed:30121295). Interacts with the hexon-interlacing protein IX; this interaction lashes the hexons together (PubMed:28978703, PubMed:30121295). Interacts with pre-protein VI; this interaction probably allows nuclear import of hexon trimers and possibly pre-capsid assembly (PubMed:14633984). Interacts with endosome lysis protein (PubMed:28978703). Interacts with pVIn (PubMed:28978703, PubMed:30121295). Interacts with pVIIn2 (PubMed:28978703). Interacts with host dyneins DYNC1LI1 and DYNC1I2; this interaction might be involved in intracellular microtubule-dependent transport of incoming viral capsid (PubMed:20006841). Interacts with the shutoff protein; this interaction allows folding and formation of hexons trimers. Interacts with host NUP214 (via N-terminus); this interaction might be essential for the release of the virus genome to the nucleus (By similarity) (PubMed:25410864). Interacts with host LTF/Lactoferrin (via N-terminus); this interaction seems to be part of the mechanisms of cellular entry by the virus (PubMed:38323814). Interacts with host blood coagulation factor F10 (via Gla domain); this interaction allows the virus to be shielded from its host immune system (PubMed:39528527)

Gene: L3  |  Organism: Human adenovirus C serotype 5  |  Synonyms: Protein II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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