Recombinant Human adenovirus C serotype 5 Protease (L3), Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human adenovirus C serotype 5 Protease (L3)

Recombinant Human adenovirus C serotype 5 Protease (L3) — Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Purity >90%.

SKU: BCRECP-003235 Categories: , , ,
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Product Specifications

Product SkuBCRECP-003235
Product DescriptionRecombinant Human adenovirus C serotype 5 Protease (L3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-204aa. [Accession | P03253].
Uniprot No.P03253
Gene NamesL3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-204aa
SpeciesHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating.

Biological Context

Subcellular Location: Virion; Host nucleus

Product Specifications

Recombinant Human adenovirus C serotype 5 Protease (L3) is a recombinant protein from Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), expressed in E.coli, covering amino acids 1-204aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating. Without maturation, the particle lacks infectivity and is unable to uncoat. Late in adenovirus infection, in the cytoplasm, may participate in the cytoskeleton destruction. Cleaves host cell cytoskeletal keratins K7 and K18

Subcellular Location

Virion; Host nucleus

Subunit

Interacts with protease cofactor pVI-C; this interaction is necessary for protease activation

Gene: L3  |  Organism: Human adenovirus C serotype 5  |  Synonyms: Adenain; Adenovirus protease; Adenovirus proteinase; Endoprotease

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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