Recombinant Human AP-1 complex subunit beta-1 (AP1B1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 73.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human AP-1 complex subunit beta-1 (AP1B1), partial

Recombinant Human AP-1 complex subunit beta-1 (AP1B1), partial — Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. Purity >85%.

SKU: BCRECP-000096 Category:
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Product Specifications

Product SkuBCRECP-000096
Product DescriptionRecombinant Human AP-1 complex subunit beta-1 (AP1B1) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 1-584aa. [Accession | Q10567].
Uniprot No.Q10567
Gene NamesAP1B1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-584aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight73.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Biological Context

Subcellular Location: Golgi apparatus; Cytoplasmic vesicle, clathrin-coated vesicle membrane (Peripheral membrane protein)
Tissue Specificity: Widely expressed
Disease Association: Keratitis-ichthyosis-deafness syndrome, autosomal recessive (KIDAR) : An autosomal recessive form of keratitis-ichthyosis-deafness syndrome, a disease characterized by the association of hyperkeratotic skin lesions with vascularizing keratitis and profound sensorineural hearing loss. KIDAR patients manifest ichthyosis, failure to thrive and developmental delay in childhood, thrombocytopenia, photophobia, and progressive hearing loss. Low plasma copper and ceruloplasmin levels have been reported in some patients. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human AP-1 complex subunit beta-1 (AP1B1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-584aa, with N-terminal 6xHis-tagged and C-terminal Myc-tagged tag, molecular weight 73.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes (PubMed:31630791). The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Subcellular Location

Golgi apparatus; Cytoplasmic vesicle, clathrin-coated vesicle membrane (Peripheral membrane protein)

Disease Association

Keratitis-ichthyosis-deafness syndrome, autosomal recessive (KIDAR) : An autosomal recessive form of keratitis-ichthyosis-deafness syndrome, a disease characterized by the association of hyperkeratotic skin lesions with vascularizing keratitis and profound sensorineural hearing loss. KIDAR patients manifest ichthyosis, failure to thrive and developmental delay in childhood, thrombocytopenia, photophobia, and progressive hearing loss. Low plasma copper and ceruloplasmin levels have been reported in some patients. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Widely expressed

Subunit

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3)

Gene: AP1B1  |  Organism: Homo sapiens  |  Synonyms: Adaptor protein complex AP-1 subunit beta-1; Adaptor-related protein complex 1 subunit beta-1; Beta-1-adaptin; Beta-adaptin 1; Clathrin assembly protein complex 1 beta large chain; Golgi adaptor HA1/AP1 adaptin beta subunit
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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