Recombinant Human Aquaporin-5 (AQP5), Nanodisc Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 29.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Aquaporin-5 (AQP5), Nanodisc Protein

Recombinant Human Aquaporin-5 (AQP5), Nanodisc Protein — Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Purity >85%.

SKU: BCRECP-00393TM Categories: ,
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Product Specifications

Uniprot No.P55064
Gene NamesAQP5
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-265aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight29.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity.

Biological Context

Subcellular Location: Apical cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein)
Tissue Specificity: Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level)
Disease Association: Keratoderma, palmoplantar, Bothnian type (PPKB) : A dermatological disorder characterized by diffuse non-epidermolytic hyperkeratosis of the skin of palms and soles. PPKB is frequently complicated by fungal infections. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Aquaporin-5 (AQP5), Nanodisc Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-265aa, with N-terminal 10xHis-tagged tag, molecular weight 29.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (PubMed:18768791, PubMed:8621489). Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity)

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein)

Disease Association

Keratoderma, palmoplantar, Bothnian type (PPKB) : A dermatological disorder characterized by diffuse non-epidermolytic hyperkeratosis of the skin of palms and soles. PPKB is frequently complicated by fungal infections. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level)

Subunit

Homotetramer; each monomer provides an independent water pore (PubMed:18768791, PubMed:26569106). Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity

Gene: AQP5  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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