Recombinant Human Arginase-1 (ARG1) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 36.2 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Arginase-1 (ARG1) (Active)

Recombinant Human Arginase-1 (ARG1) (Active) — Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Purity >95%.

SKU: BCACP-000620 Category:
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Product SKUBCACP-000620
Product DescriptionRecombinant Human Arginase-1 (ARG1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1-322aa. [Accession | P05089].
Uniprot No.P05089
Gene NamesARG1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1-322aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight36.2 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis NT5E at 2 μg/mL can bind anti-NT5E recombinant antibody.The EC50 is 0.6454-0.7583 ng/mL.;NT5E Recombinant Monoclonal Antibody captured on Protein A Chip can bind Macaca fascicularis NT5E with an affinity constant of 0.253 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesArginase-1; EC 3.5.3.1; Liver-type arginase; Type I arginase

Function

Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN).

Biological Context

Subcellular Location: Cytoplasm; Cytoplasmic granule
Tissue Specificity: Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease
Disease Association: Argininemia (ARGIN) : A rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, intellectual disability, hypotonia, ataxia and progressive spastic quadriplegia. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1

Product Specifications

Recombinant Human Arginase-1 (ARG1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-322aa, with C-terminal 10xHis-tagged tag, molecular weight 36.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis NT5E at 2 μg/mL can bind anti-NT5E recombinant antibody.The EC50 is 0.6454-0.7583 ng/mL.;NT5E Recombinant Monoclonal Antibody captured on Protein A Chip can bind Macaca fascicularis NT5E with an affinity constant of 0.253 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure

Subcellular Location

Cytoplasm; Cytoplasmic granule

Disease Association

Argininemia (ARGIN) : A rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, intellectual disability, hypotonia, ataxia and progressive spastic quadriplegia. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) (PubMed:15546957). Also detected in macrophages mycobacterial granulomas (PubMed:23749634). Expressed in group2 innate lymphoid cells (ILC2s) during lung disease (PubMed:27043409)

Subunit

Homotrimer (PubMed:16141327, PubMed:17469833, PubMed:17562323, PubMed:18802628, PubMed:2241902). Interacts with CMTM6 (PubMed:28813417)

Pathway

Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1

Gene: ARG1  |  Organism: Homo sapiens  |  Synonyms: Liver-type arginase; Type I arginase
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