Recombinant Human ATP-dependent RNA helicase DDX1 (DDX1), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 89.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human ATP-dependent RNA helicase DDX1 (DDX1)

Recombinant Human ATP-dependent RNA helicase DDX1 (DDX1) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-000489 Category:
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Product Specifications

Product SkuBCRECP-000489
Product DescriptionRecombinant Human ATP-dependent RNA helicase DDX1 (DDX1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-740aa. [Accession | Q92499].
Uniprot No.Q92499
Gene NamesDDX1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-740aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight89.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

(Microbial infection) Required for Coronavirus IBV replication.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord

Product Specifications

Recombinant Human ATP-dependent RNA helicase DDX1 (DDX1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-740aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 89.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

(Microbial infection) Required for Coronavirus IBV replication

Subcellular Location

Cytoplasm

Tissue Specificity

Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord

Subunit

(Microbial infection) Interacts with Severe acute respiratory syndrome coronavirus (SARS-CoV) (via N-terminus) (PubMed:20573827). Interacts (via C-terminus) with the replicase polyprotein 1ab Nsp14 of the Avian infectious bronchitis virus (IBV)

Gene: DDX1  |  Organism: Homo sapiens  |  Synonyms: DEAD box protein 1; DEAD box protein retinoblastoma
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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