Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-Myc-tagged, 43.9 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active)

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active) — Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. Purity >90%.

SKU: BCACP-000037 Category:
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Product SKUBCACP-000037
Product DescriptionRecombinant Human B- and T-lymphocyte attenuator (BTLA) protein is expressed from Mammalian cell with C-terminal hFc1-Myc-tagged. It contains 31-150aa. [Accession | Q7Z6A9].
Uniprot No.Q7Z6A9
Gene NamesBTLA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region31-150aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-Myc-tagged
Molecular weight43.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14 , the EC50 is 137.8-233.4 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(B- and T-lymphocyte-associated protein)(CD272)

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 31-150aa, with C-terminal hFc1-Myc-tagged tag, molecular weight 43.9 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14 , the EC50 is 137.8-233.4 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006). Interacts with TNFRSF14/HVEM (via cysteine-rich domain 1) (PubMed:15568026, PubMed:18193050, PubMed:19915044)

Gene: BTLA  |  Organism: Homo sapiens  |  Synonyms: B- and T-lymphocyte-associated protein
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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