Recombinant Human Carbonic anhydrase 9 (CA9), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-GST-tagged, 66.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Carbonic anhydrase 9 (CA9), partial

Recombinant Human Carbonic anhydrase 9 (CA9), partial — Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i. Purity >85%.

SKU: BCRECP-000207 Category:
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Product Specifications

Product SkuBCRECP-000207
Product DescriptionRecombinant Human Carbonic anhydrase 9 (CA9) Protein is expressed from E.coli with N-terminal 6xHis-GST-tagged. It contains 64-399aa. [Accession | Q16790].
Uniprot No.Q16790
Gene NamesCA9
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region64-399aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-GST-tagged
Molecular weight66.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Biological Context

Subcellular Location: Nucleus; Nucleus, nucleolus; Cell membrane (Single-pass type I membrane protein); Cell projection, microvillus membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa

Product Specifications

Recombinant Human Carbonic anhydrase 9 (CA9), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 64-399aa, with N-terminal 6xHis-GST-tagged tag, molecular weight 66.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions)

Subcellular Location

Nucleus; Nucleus, nucleolus; Cell membrane (Single-pass type I membrane protein); Cell projection, microvillus membrane (Single-pass type I membrane protein)

Tissue Specificity

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa

Subunit

Forms oligomers linked by disulfide bonds

Gene: CA9  |  Organism: Homo sapiens  |  Synonyms: Carbonate dehydratase IX; Carbonic anhydrase IX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; pMW1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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