Recombinant Human Caspase-5 (CASP5), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 37.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Caspase-5 (CASP5), partial

Recombinant Human Caspase-5 (CASP5), partial — Thiol protease that acts as a mediator of programmed cell death. Purity >90%.

SKU: BCRECP-000238 Category:
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Product Specifications

Product SkuBCRECP-000238
Product DescriptionRecombinant Human Caspase-5 (CASP5) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 137-327aa. [Accession | P51878].
Uniprot No.P51878
Gene NamesCASP5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region137-327aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight37.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Thiol protease that acts as a mediator of programmed cell death. Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. Also mediates cleavage and maturation of IL18.

Biological Context

Tissue Specificity: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle

Product Specifications

Recombinant Human Caspase-5 (CASP5), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 137-327aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 37.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Thiol protease that acts as a mediator of programmed cell death (PubMed:28314590, PubMed:29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893). Also mediates cleavage and maturation of IL18 (PubMed:37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed:37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590)

Tissue Specificity

Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle

Subunit

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits (By similarity). Interacts with MEFV (PubMed:17431422). Interacts with SERPINB1; this interaction regulates CASP5 activity (PubMed:30692621)

Gene: CASP5  |  Organism: Homo sapiens  |  Synonyms: ICE(rel)-III; Protease ICH-3; Protease TY
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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