Recombinant Human Caspase-7 (CASP7), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 23.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Caspase-7 (CASP7), partial

Recombinant Human Caspase-7 (CASP7), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >90%.

SKU: BCRECP-000239 Category:
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Product Specifications

Product SkuBCRECP-000239
Product DescriptionRecombinant Human Caspase-7 (CASP7) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 24-198aa. [Accession | P55210].
Uniprot No.P55210
Gene NamesCASP7
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-198aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight23.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasApoptosis

Function

Lacks enzymatic activity.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Nucleus; Secreted, extracellular space
Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain

Product Specifications

Recombinant Human Caspase-7 (CASP7), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 24-198aa, with N-terminal 6xHis-tagged tag, molecular weight 23.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Lacks enzymatic activity

Subcellular Location

Cytoplasm, cytosol; Nucleus; Secreted, extracellular space

Tissue Specificity

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain

Subunit

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit (PubMed:11701129, PubMed:11752425, PubMed:16916640, PubMed:20566630). Interacts with XIAP (via its second BIR domain); inhibiting CASP7 activity (PubMed:11257230, PubMed:11257231, PubMed:16916640). Interacts with BIRC6/bruce (PubMed:15200957). Interacts with ATXN3 (short isoform 1) (PubMed:30455355). Interacts with HSPA5 (PubMed:26045166)

Gene: CASP7  |  Organism: Homo sapiens  |  Synonyms: Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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