Recombinant Human Cathepsin D (CTSD) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 44.0 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Cathepsin D (CTSD) (Active)

Recombinant Human Cathepsin D (CTSD) (Active) — Acid protease active in intracellular protein breakdown. Purity >95%.

SKU: BCACP-000615 Category:
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Product SKUBCACP-000615
Product DescriptionRecombinant Human Cathepsin D (CTSD) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 21-412aa. [Accession | P07339].
Uniprot No.P07339
Gene NamesCTSD
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region21-412aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight44.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TFRC at 2 μg/mL can bind Anti-TFRC recombinant antibody . The EC50 is 19.84-27.47 ng/mL.;TFRC Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis TFRC with an affinity constant of 0.28 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCathepsin D; EC 3.4.23.5 [Cleaved into: Cathepsin D light chain; Cathepsin D heavy chain]

Function

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Biological Context

Subcellular Location: Lysosome; Melanosome; Secreted, extracellular space
Tissue Specificity: Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level)
Disease Association: Ceroid lipofuscinosis, neuronal, 10 (CLN10) : A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Cathepsin D (CTSD) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 21-412aa, with C-terminal 10xHis-tagged tag, molecular weight 44.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease

Subcellular Location

Lysosome; Melanosome; Secreted, extracellular space

Disease Association

Ceroid lipofuscinosis, neuronal, 10 (CLN10) : A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in the aorta extracellular space (at protein level) (PubMed:20551380). Expressed in liver (at protein level) (PubMed:1426530)

Subunit

Consists of a light chain and a heavy chain (PubMed:1426530, PubMed:8393577). Interacts with ADAM30; this leads to activation of CTSD (PubMed:27333034). Interacts with GRN; stabilizes CTSD; increases its proteolytic activity (By similarity)

Gene: CTSD  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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