Recombinant Human Cathepsin K (CTSK), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 27.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Cathepsin K (CTSK)

Recombinant Human Cathepsin K (CTSK) — Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Purity >90%.

SKU: BCRECP-000456 Category:
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Product Specifications

Product SkuBCRECP-000456
Product DescriptionRecombinant Human Cathepsin K (CTSK) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 115-329aa. [Accession | P43235].
Uniprot No.P43235
Gene NamesCTSK
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region115-329aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight27.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

Biological Context

Subcellular Location: Lysosome; Secreted; Apical cell membrane (Peripheral membrane protein)
Tissue Specificity: Predominantly expressed in osteoclasts (bones). Expressed in thyroid epithelial cells
Disease Association: Pycnodysostosis (PKND) : A rare autosomal recessive bone disorder characterized by deformity of the skull, maxilla and phalanges, osteosclerosis, and fragility of bone. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Cathepsin K (CTSK) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 115-329aa, with N-terminal 6xHis-tagged tag, molecular weight 27.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed:11082042)

Subcellular Location

Lysosome; Secreted; Apical cell membrane (Peripheral membrane protein)

Disease Association

Pycnodysostosis (PKND) : A rare autosomal recessive bone disorder characterized by deformity of the skull, maxilla and phalanges, osteosclerosis, and fragility of bone. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Predominantly expressed in osteoclasts (bones) (PubMed:7805878). Expressed in thyroid epithelial cells (PubMed:11082042)

Gene: CTSK  |  Organism: Homo sapiens  |  Synonyms: Cathepsin O; Cathepsin O2; Cathepsin X
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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