Recombinant Human CD48 antigen (CD48) (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 51.3 kDa, purity >90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
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Recombinant Human CD48 antigen (CD48) (Active)

Recombinant Human CD48 antigen (CD48) (Active) — Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immu… Purity >90%.

SKU: BCACP-000064 Categories: ,
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Product SKUBCACP-000064
Product DescriptionRecombinant Human CD48 antigen (CD48) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 27-220aa. [Accession | P09326].
Uniprot No.P09326
Gene NamesCD48
PurityGreater than 90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region27-220aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight51.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 μg/ml can bind human BCMA , the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.;Human TNFSF13B protein Fc tag captured on COOH chip can bind Human BCMA protein Fc tag with an affinity constant of 39 nM as detected by LSPR Assay.;Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C, the EC50 is 9.943-15.72 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesB-lymphocyte activation marker BLAST-1 (BCM1 surface antigen) (Leukocyte antigen MEM-102) (SLAM family member 2) (SLAMF2) (Signaling lymphocytic activation molecule 2) (TCT.1) (CD48) (BCM1) (BLAST1)

Function

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation. Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn, promotes LCK phosphorylation and subsequent activation.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor); Membrane raft; Secreted
Tissue Specificity: Widely expressed on all hematopoietic cells

Product Specifications

Recombinant Human CD48 antigen (CD48) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 27-220aa, with C-terminal hFc1-tagged tag, molecular weight 51.3 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 μg/ml can bind human BCMA , the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.;Human TNFSF13B protein Fc tag captured on COOH chip can bind Human BCMA protein Fc tag with an affinity constant of 39 nM as detected by LSPR Assay.;Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C, the EC50 is 9.943-15.72 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor); Membrane raft; Secreted

Tissue Specificity

Widely expressed on all hematopoietic cells

Subunit

Interacts with CD2 (PubMed:19494291). Interacts with CD244; this interaction is possible not only on different cells (trans interaction) but also on the same cell (cis interaction) (PubMed:27249817, PubMed:9841922). Interacts with LCK (PubMed:12007789)

Gene: CD48  |  Organism: Homo sapiens  |  Synonyms: B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; Signaling lymphocytic activation molecule 2; TCT.1
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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