Recombinant Human CD70 antigen (CD70), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal 10xHis-tagged, 22.7 kDa, purity >92.5% as determined by SDS-PAGE.
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Recombinant Human CD70 antigen (CD70), partial (Active)

Recombinant Human CD70 antigen (CD70), partial (Active) — Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. Purity >92.5%.

SKU: BCACP-000233 Categories: ,
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Product SKUBCACP-000233
Product DescriptionRecombinant Human CD70 antigen (CD70) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 52-193aa. [Accession | P32970].
Uniprot No.P32970
Gene NamesCD70
PurityGreater than 92.5% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region52-193aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight22.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Mouse CADM1 at 2 μg/mL can bind Mouse CRTAM , the EC50 is 52.61-92.92 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(CD27 ligand) (CD27-L)(CD27L)(CD27LG)(TNFSF7)

Function

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.

Product Specifications

Recombinant Human CD70 antigen (CD70), partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 52-193aa, with N-terminal 10xHis-tagged tag, molecular weight 22.7 kDa, purity Greater than 92.5% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864)

Gene: CD70  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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