Recombinant Human Clathrin heavy chain 2 (CLTCL1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 20.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Clathrin heavy chain 2 (CLTCL1), partial

Recombinant Human Clathrin heavy chain 2 (CLTCL1), partial — Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Purity >90%.

SKU: BCRECP-000386 Category:
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Product Specifications

Product SkuBCRECP-000386
Product DescriptionRecombinant Human Clathrin heavy chain 2 (CLTCL1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1423-1566aa. [Accession | P53675].
Uniprot No.P53675
Gene NamesCLTCL1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1423-1566aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight20.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

Biological Context

Subcellular Location: Cytoplasmic vesicle membrane (Peripheral membrane protein); Membrane, coated pit (Peripheral membrane protein)
Tissue Specificity: Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues

Product Specifications

Recombinant Human Clathrin heavy chain 2 (CLTCL1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1423-1566aa, with N-terminal 6xHis-tagged tag, molecular weight 20.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity)

Subcellular Location

Cytoplasmic vesicle membrane (Peripheral membrane protein); Membrane, coated pit (Peripheral membrane protein)

Tissue Specificity

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues

Subunit

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (By similarity). In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (Probable). May interact with OCRL (By similarity). Interacts with AFTPH/aftiphilin (PubMed:15758025)

Gene: CLTCL1  |  Organism: Homo sapiens  |  Synonyms: Clathrin heavy chain on chromosome 22
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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