Recombinant Human Claudin-3 (CLDN3)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions), 24.7 kDa
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Recombinant Human Claudin-3 (CLDN3)-VLPs (Active)

Recombinant Human Claudin-3 (CLDN3)-VLPs (Active) — Barrier-forming claudin.

SKU: BCACP-000171 Categories: ,
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Product SKUBCACP-000171
Product DescriptionRecombinant Human Claudin-3 (CLDN3)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions). It contains 1-220aa. [Accession | O15551].
Uniprot No.O15551
Gene NamesCLDN3
Expression SystemMammalian cell
Expression Region1-220aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular weight24.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human RSPO1 at 2 μg/mL can bind Human LGR5, the EC50 is 124.0-174.1 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(Clostridium perfringens enterotoxin receptor 2)(CPE-R 2; CPE-receptor 2)(Rat ventral prostate.1 protein homolog)(hRVP1)

Function

Barrier-forming claudin. Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Biological Context

Subcellular Location: Cell junction, tight junction; Cell membrane (Multi-pass membrane protein)
Disease Association: [CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region]

Product Specifications

Recombinant Human Claudin-3 (CLDN3)-VLPs (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-220aa, with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) tag, molecular weight 24.7 kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Barrier-forming claudin. Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity

Subcellular Location

Cell junction, tight junction; Cell membrane (Multi-pass membrane protein)

Disease Association

[CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region]

Subunit

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN1 and CLDN2 homopolymers. Interacts in cis (within the same plasma membrane) with CLDN19 (PubMed:28028216, PubMed:36008380). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity)

Gene: CLDN3  |  Organism: Homo sapiens  |  Synonyms: Clostridium perfringens enterotoxin receptor 2; Rat ventral prostate.1 protein homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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