Recombinant Human Clusterin (CLU), Homo sapiens (Human), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 70.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Clusterin (CLU)

Recombinant Human Clusterin (CLU) — Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Purity >85%.

SKU: BCRECP-000387 Category:
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Product Specifications

Product SkuBCRECP-000387
Product DescriptionRecombinant Human Clusterin (CLU) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 23-449aa. [Accession | P10909].
Uniprot No.P10909
Gene NamesCLU
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region23-449aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight70.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX.

Biological Context

Subcellular Location: Nucleus; Cytoplasm; Mitochondrion membrane (Peripheral membrane protein); Cytoplasm, cytosol; Microsome; Endoplasmic reticulum; Mitochondrion; Mitochondrion membrane; Cytoplasm, perinuclear region; Cytoplasmic vesicle, secretory vesicle, chromaffin granule
Tissue Specificity: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung

Product Specifications

Recombinant Human Clusterin (CLU) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 23-449aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 70.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed:24073260). Promotes cell death through interaction with BCL2L1 that releases and activates BAX (PubMed:21567405)

Subcellular Location

Nucleus; Cytoplasm; Mitochondrion membrane (Peripheral membrane protein); Cytoplasm, cytosol; Microsome; Endoplasmic reticulum; Mitochondrion; Mitochondrion membrane; Cytoplasm, perinuclear region; Cytoplasmic vesicle, secretory vesicle, chromaffin granule

Tissue Specificity

Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung

Subunit

Antiparallel disulfide-linked heterodimer of an alpha chain and a beta chain (PubMed:12047389, PubMed:1491011, PubMed:1551440, PubMed:2387851, PubMed:2780565, PubMed:8328966). Self-associates and forms higher oligomers (PubMed:1903064). Interacts with a broad range of misfolded proteins, including APP, APOC2 and LYZ (PubMed:17407782, PubMed:17412999, PubMed:8328966). Slightly acidic pH promotes interaction with misfolded proteins (PubMed:12176985). Forms high-molecular weight oligomers upon interaction with misfolded proteins (PubMed:19535339). Interacts with APOA1, LRP2, CLUAP1 and PON1 (PubMed:15480429, PubMed:17260971, PubMed:1742316, PubMed:1903064, PubMed:8292612). Interacts with the complement membrane attack complex (PubMed:2601725, PubMed:9200695, PubMed:34667172). Interacts (via alpha chain) with XRCC6 (By similarity). Interacts with SYVN1, COMMD1, BTRC, CUL1 and with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes (PubMed:17451556, PubMed:20068069). Interacts (via alpha chain) with BAX in stressed cells, where BAX undergoes a conformation change leading to association with the mitochondrial membrane (PubMed:16113678). Does not interact with BAX in unstressed cells (PubMed:16113678). Found in a complex with LTF, CLU, EPPIN and SEMG1 (PubMed:17567961). Interacts (immaturely glycosylated pre-secreted form) with HSPA5; this interaction promotes CLU stability and facilitates stress-induced CLU retrotranslocation from the secretory pathway to the mitochondria, thereby reducing stress-induced apoptosis by stabilizing mitochondrial membrane integrity (PubMed:22689054). Interacts (isoform 4) with BCL2L1; this interaction releases and activates BAX and promotes cell death (PubMed:21567405). Interacts with TGFBR2 and ACVR1 (PubMed:8555189). Interacts (secreted form) with STMN3; this interaction may act as an important modulator during neuronal differentiation (By similarity). Interacts with VLDLR and LRP8 (PubMed:24381170)

Gene: CLU  |  Organism: Homo sapiens  |  Synonyms: Aging-associated gene 4 protein; Apolipoprotein J; Complement cytolysis inhibitor; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Sulfated glycoprotein 2; Testosterone-repressed prostate message 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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