Recombinant Human Complement factor D(CFD) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 25.9 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Complement factor D(CFD) (Active)

Recombinant Human Complement factor D(CFD) (Active) — Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adapt… Purity >95%.

SKU: BCACP-000485 Category:
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Product SKUBCACP-000485
Product DescriptionRecombinant Human Complement factor D(CFD) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 26-253aa. [Accession | P00746].
Uniprot No.P00746
Gene NamesCFD
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region26-253aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight25.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis NCR3LG1 at 5 μg/mL on an Nickel Coated plate can bind Macaca fascicularis NCR3. The EC50 is 1.171-1.377 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesComplement factor D; EC:3.4.21.46; Adipsin; C3 convertase activator; Properdin factor D; CFD;DF, PFD

Function

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases.

Product Specifications

Recombinant Human Complement factor D(CFD) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 26-253aa, with C-terminal 10xHis-tagged tag, molecular weight 25.9 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis NCR3LG1 at 5 μg/mL on an Nickel Coated plate can bind Macaca fascicularis NCR3. The EC50 is 1.171-1.377 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:21205667, PubMed:22362762, PubMed:6769474, PubMed:874324, PubMed:9748277). In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3 (PubMed:21205667, PubMed:22362762, PubMed:6769474, PubMed:874324). The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases (PubMed:21205667, PubMed:22362762, PubMed:6769474, PubMed:874324). Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway (PubMed:21205667, PubMed:6769474, PubMed:874324, PubMed:9748277)

Gene: CFD  |  Organism: Homo sapiens

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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