Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial, Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5), E.coli, N-terminal 6xHis-tagged, 26.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial

Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial — The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Purity >85%.

SKU: BCRECP-002846 Categories: ,
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Product Specifications

Product SkuBCRECP-002846
Product DescriptionRecombinant Human cytomegalovirus Envelope glycoprotein H (gH) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 24-195aa. [Accession | P12824].
Uniprot No.P12824
Gene NamesgH
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-195aa
SpeciesHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Tag InfoN-terminal 6xHis-tagged
Molecular weight26.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial is a recombinant protein from Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5), expressed in E.coli, covering amino acids 24-195aa, with N-terminal 6xHis-tagged tag, molecular weight 26.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis (By similarity). In human cytomegalovirus, forms two distinct complexes to mediate viral entry, a trimer and a pentamer at the surface of the virion envelope. The gH-gL-gO trimer is required for infection in fibroblasts by interacting with host PDGFRA, and in glioblastoma cells by interacting with host EPHA2. The gH-gL-UL128-UL130-UL131A pentamer is essential for viral entry in epithelial, endothelial and myeloid cells via interaction with host NRP2 (By similarity)

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein); Host endosome membrane (Single-pass type I membrane protein)

Subunit

Interacts with glycoprotein L (gL); this interaction is necessary for the correct processing and cell surface expression of gH. The heterodimer gH/gL seems to interact with gB trimers during fusion (By similarity). Forms the envelope pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A. The pentamer interacts with host NRP2. Forms the envelope trimer complex composed of gH, gL, and gO (PubMed:9733861). The trimer interacts with host PDGFRA (By similarity). The trimer also interacts with host EPHA2 (By similarity). Interacts with UL116 (By similarity)

Gene: gH  |  Organism: Human cytomegalovirus (strain AD169)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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