Recombinant Human cytomegalovirus Large structural phosphoprotein (UL32), partial, Biotinylated, Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 81.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human cytomegalovirus Large structural phosphoprotein (UL32), partial, Biotinylated

Recombinant Human cytomegalovirus Large structural phosphoprotein (UL32), partial, Biotinylated — Participates in the last steps of viral maturation and release. Purity >90%.

SKU: BCRECP-004682 Categories: , ,
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Product Specifications

Product SkuBCRECP-004682
Product DescriptionRecombinant Human cytomegalovirus Large structural phosphoprotein (UL32) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 1-285aa. [Accession | P08318].
Uniprot No.P08318
Gene NamesUL32
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-285aa
SpeciesHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight81.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Participates in the last steps of viral maturation and release. Associates with nuclear capsids prior to DNA encapsidation and later preserves the integrity of nucleocapsids through secondary envelopment at the assembly compartment. Interacts with host CCNA2 and thereby blocks the onset of lytic gene expression to promote establishment of a quiescent state of infection in undifferentiated cells.

Biological Context

Subcellular Location: Virion tegument; Host cytoplasm; Host nucleus

Product Specifications

Recombinant Human cytomegalovirus Large structural phosphoprotein (UL32), partial, Biotinylated is a recombinant protein from Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5), expressed in E.coli, covering amino acids 1-285aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 81.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Participates in the last steps of viral maturation and release. Associates with nuclear capsids prior to DNA encapsidation and later preserves the integrity of nucleocapsids through secondary envelopment at the assembly compartment. Interacts with host CCNA2 and thereby blocks the onset of lytic gene expression to promote establishment of a quiescent state of infection in undifferentiated cells

Subcellular Location

Virion tegument; Host cytoplasm; Host nucleus

Subunit

Interacts with host BICD1 and RAB6A. Interacts with small capsid protein UL48A; this interaction links together the capsid and pp150. Interacts with host CCNA2

Gene: UL32  |  Organism: Human cytomegalovirus (strain AD169)  |  Synonyms: 150 kDa matrix phosphoprotein; 150 kDa phosphoprotein; Basic phosphoprotein; Phosphoprotein UL32; Tegument protein UL32
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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