Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 42.5 kDa, purity >90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
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Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active)

Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active) — Inhibitory receptor acting as a major negative regulator of T-cell responses. Purity >90%.

SKU: BCACP-000065 Categories: ,
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Product SKUBCACP-000065
Product DescriptionRecombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 37-162aa. [Accession | P16410].
Uniprot No.P16410
Gene NamesCTLA4
PurityGreater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region37-162aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight42.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1, the EC50 of human ULBP1 protein is 228.5-427.6 ng/ml.;Human KLRK1 protein Fc tag captured on COOH chip can bind Human ULBP1 protein Fc/myc tag with an affinity constant of 2.27 nM as detected by LSPR Assay.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCTLA4; CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152

Function

Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation
Disease Association: Immune dysregulation with autoimmunity, immunodeficiency, and lymphoproliferation (IDAIL) : An autosomal dominant primary immunodeficiency characterized by severe autoimmunity, infiltration of non-lymphoid organs, such as the intestine, lungs and brain, by hyperactive T cells and B cells, autoimmune cytopenias, and hypogammaglobulinemia in early childhood. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Cytotoxic T-lymphocyte protein 4 (CTLA4), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 37-162aa, with C-terminal hFc1-tagged tag, molecular weight 42.5 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1, the EC50 of human ULBP1 protein is 228.5-427.6 ng/ml.;Human KLRK1 protein Fc tag captured on COOH chip can bind Human ULBP1 protein Fc/myc tag with an affinity constant of 2.27 nM as detected by LSPR Assay.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed:11279501, PubMed:11279502, PubMed:16551244, PubMed:1714933, PubMed:18641304, PubMed:28484017). Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed:11279501, PubMed:11279502, PubMed:16551244, PubMed:1714933, PubMed:28484017)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Disease Association

Immune dysregulation with autoimmunity, immunodeficiency, and lymphoproliferation (IDAIL) : An autosomal dominant primary immunodeficiency characterized by severe autoimmunity, infiltration of non-lymphoid organs, such as the intestine, lungs and brain, by hyperactive T cells and B cells, autoimmune cytopenias, and hypogammaglobulinemia in early childhood. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation

Subunit

Homodimer; disulfide-linked (PubMed:11279501, PubMed:11279502, PubMed:21156796, PubMed:28484017, Ref.24). Interacts with ICOSLG (PubMed:28484017)

Gene: CTLA4  |  Organism: Homo sapiens  |  Synonyms: Cytotoxic T-lymphocyte-associated antigen 4
Key Publications

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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