Recombinant Human DDB1- and CUL4-associated factor 7 (DCAF7), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 45.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human DDB1- and CUL4-associated factor 7 (DCAF7)

Recombinant Human DDB1- and CUL4-associated factor 7 (DCAF7) — Involved in craniofacial development. Purity >85%.

SKU: BCRECP-000475 Category:
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Product Specifications

Product SkuBCRECP-000475
Product DescriptionRecombinant Human DDB1- and CUL4-associated factor 7 (DCAF7) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-342aa. [Accession | P61962].
Uniprot No.P61962
Gene NamesDCAF7
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-342aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight45.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human DDB1- and CUL4-associated factor 7 (DCAF7) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-342aa, with N-terminal 10xHis-tagged tag, molecular weight 45.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex

Subcellular Location

Cytoplasm; Nucleus

Subunit

Interacts with DYRK1A, DYRK1B and DIAPH1. Interacts with DDB1. Interacts with ZNF703. Interacts with human adenovirus 5 E1A protein (PubMed:23864635)

Pathway

Protein modification; protein ubiquitination

Gene: DCAF7  |  Organism: Homo sapiens  |  Synonyms: WD repeat-containing protein 68; WD repeat-containing protein An11 homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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