Recombinant Human Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33), partial, Homo sapiens (Human), Baculovirus, C-terminal 6xHis-tagged, 78.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33), partial

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33), partial — Metalloprotease that cleaves and sheds the ectodomains of cell-surface proteins and thereby contributes to angiogenesis, cell differentiation, inflammation, and cell proliferation. Purity >90%.

SKU: BCRECP-000032 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-000032
Product DescriptionRecombinant Human Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33) Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 30-701aa. [Accession | Q9BZ11].
Uniprot No.Q9BZ11
Gene NamesADAM33
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region30-701aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight78.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Metalloprotease that cleaves and sheds the ectodomains of cell-surface proteins and thereby contributes to angiogenesis, cell differentiation, inflammation, and cell proliferation. Modulates the activity of adhesion molecules and cytokines by generating soluble forms, leading to either attenuation of cell-surface signaling or initiation of downstream intracellular signaling pathways. For instance, acts as a constitutive sheddase of kit ligand/KITLG.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins
Disease Association: Asthma (ASTHMA) : The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with wheezing due to spasmodic contraction of the bronchi. [Disease susceptibility is associated with variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33), partial is a recombinant protein from Homo sapiens (Human), expressed in Baculovirus, covering amino acids 30-701aa, with C-terminal 6xHis-tagged tag, molecular weight 78.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Metalloprotease that cleaves and sheds the ectodomains of cell-surface proteins and thereby contributes to angiogenesis, cell differentiation, inflammation, and cell proliferation (PubMed:14676211, PubMed:33982767). Modulates the activity of adhesion molecules and cytokines by generating soluble forms, leading to either attenuation of cell-surface signaling or initiation of downstream intracellular signaling pathways. For instance, acts as a constitutive sheddase of kit ligand/KITLG (PubMed:14676211). Promotes and maintains a proliferative phenotype in vascular smooth muscle cells through regulation of the PI3K/AKT and ERK signaling pathways (PubMed:33982767). Additionally, enhances cell migration and regulates cytokine secretion during airway vascular remodeling via the same signaling cascades (PubMed:36091328)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Disease Association

Asthma (ASTHMA) : The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with wheezing due to spasmodic contraction of the bronchi. [Disease susceptibility is associated with variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins

Gene: ADAM33  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top