Recombinant Human DNA nucleotidylexotransferase (DNTT), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 62.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human DNA nucleotidylexotransferase (DNTT)

Recombinant Human DNA nucleotidylexotransferase (DNTT) — Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5′-triphosphate to the 3′-end of a DNA initiator. Purity >90%.

SKU: BCRECP-000526 Categories: ,
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Product Specifications

Product SkuBCRECP-000526
Product DescriptionRecombinant Human DNA nucleotidylexotransferase (DNTT) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-509aa. [Accession | P04053].
Uniprot No.P04053
Gene NamesDNTT
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-509aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight62.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Human DNA nucleotidylexotransferase (DNTT) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-509aa, with N-terminal 6xHis-tagged tag, molecular weight 62.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells

Subcellular Location

Nucleus

Subunit

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1 (PubMed:16371131)

Gene: DNTT  |  Organism: Homo sapiens  |  Synonyms: Terminal addition enzyme; Terminal deoxynucleotidyltransferase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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