Recombinant Human DNA nucleotidylexotransferase (DNTT), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 62.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human DNA nucleotidylexotransferase (DNTT)

Recombinant Human DNA nucleotidylexotransferase (DNTT) — Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5′-triphosphate to the 3′-end of a DNA initiator. Purity >90%.

SKU: BCRECP-000526 Categories: ,
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Product Specifications

Product SkuBCRECP-000526
Product DescriptionRecombinant Human DNA nucleotidylexotransferase (DNTT) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-509aa. [Accession | P04053].
Uniprot No.P04053
Gene NamesDNTT
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-509aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight62.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Human DNA nucleotidylexotransferase (DNTT) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-509aa, with N-terminal 6xHis-tagged tag, molecular weight 62.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells

Subcellular Location

Nucleus

Subunit

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1 (PubMed:16371131)

Gene: DNTT  |  Organism: Homo sapiens  |  Synonyms: Terminal addition enzyme; Terminal deoxynucleotidyltransferase
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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