Recombinant Human Dynactin subunit 4 (DCTN4), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 58.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Dynactin subunit 4 (DCTN4)

Recombinant Human Dynactin subunit 4 (DCTN4) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >90%.

SKU: BCRECP-000484 Category:
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Product Specifications

Product SkuBCRECP-000484
Product DescriptionRecombinant Human Dynactin subunit 4 (DCTN4) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-460aa. [Accession | Q9UJW0].
Uniprot No.Q9UJW0
Gene NamesDCTN4
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-460aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight58.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.

Biological Context

Subcellular Location: Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, cell cortex; Cytoplasm, myofibril, sarcomere

Product Specifications

Recombinant Human Dynactin subunit 4 (DCTN4) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-460aa, with N-terminal 6xHis-tagged tag, molecular weight 58.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules

Subcellular Location

Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, cell cortex; Cytoplasm, myofibril, sarcomere

Subunit

Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin (By similarity). Interacts with ATP7B, but not ATP7A, in a copper-dependent manner (PubMed:16554302). Interacts with ANK2; this interaction is required for localization at costameres (By similarity). Interacts with N4BP2L1 (By similarity)

Gene: DCTN4  |  Organism: Homo sapiens  |  Synonyms: Dynactin subunit p62
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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