Recombinant Human E3 ubiquitin-protein ligase RNFT1 (RNFT1) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 51.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human E3 ubiquitin-protein ligase RNFT1 (RNFT1) Protein

Recombinant Human E3 ubiquitin-protein ligase RNFT1 (RNFT1) Protein — E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for… Purity >85%.

SKU: BCRECP-00227TM Categories: ,
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Product Specifications

Uniprot No.Q5M7Z0
Gene NamesRNFT1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-435aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight51.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed at highest levels in testis, lower levels in heart, liver, lung, and kidney. Not detected in brain, ovary, and uterus. Down-regulated in testis from patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS). Ubiquitously expressed with high expression in testis
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human E3 ubiquitin-protein ligase RNFT1 (RNFT1) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-435aa, with N-terminal 10xHis-tagged tag, molecular weight 51.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not Test

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis

Subcellular Location

Endoplasmic reticulum membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed at highest levels in testis, lower levels in heart, liver, lung, and kidney (PubMed:17074343). Not detected in brain, ovary, and uterus (PubMed:17074343). Down-regulated in testis from patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS) (PubMed:17074343). Ubiquitously expressed with high expression in testis (PubMed:27485036)

Pathway

Protein modification; protein ubiquitination

Gene: RNFT1  |  Organism: Homo sapiens  |  Synonyms: Protein PTD016; RING finger and transmembrane domain-containing protein 1

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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