Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 24.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial

Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial — E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Purity >90%.

SKU: BCRECP-001670 Categories: ,
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Product Specifications

Product SkuBCRECP-001670
Product DescriptionRecombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 198-374aa. [Accession | Q9HCE7].
Uniprot No.Q9HCE7
Gene NamesSMURF1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region198-374aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight24.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.

Biological Context

Subcellular Location: Cytoplasm; Cell membrane (Peripheral membrane protein)
Tissue Specificity: Expressed in melanocytes
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 198-374aa, with N-terminal 6xHis-tagged tag, molecular weight 24.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Acts as a positive regulator of smoothened signaling pathway by mediating ubiquitination of PTCH1, leading to endocytosis and lysosomal degradation of PTCH1 (By similarity). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611). Plays a role in dendrite formation by melanocytes (PubMed:23999003)

Subcellular Location

Cytoplasm; Cell membrane (Peripheral membrane protein)

Tissue Specificity

Expressed in melanocytes (PubMed:23999003)

Subunit

Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. Interacts with MAVS; the interaction is mediated by NDFIP1 (PubMed:23087404)

Pathway

Protein modification; protein ubiquitination

Gene: SMURF1  |  Organism: Homo sapiens  |  Synonyms: HECT-type E3 ubiquitin transferase SMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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