Recombinant Human Folate receptor alpha(FOLR1),partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 25.9 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.

Recombinant Human Folate receptor alpha(FOLR1),partial (Active)

Recombinant Human Folate receptor alpha(FOLR1),partial (Active) — Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Purity >95%.

SKU: BCACP-000304 Category:
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Product SKUBCACP-000304
Product DescriptionRecombinant Human Folate receptor alpha(FOLR1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 25-233aa. [Accession | P15328].
Uniprot No.P15328
Gene NamesFOLR1
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region25-233aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight25.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human PTK7 at 2 μg/mL can bind Anti-PTK7 recombinant antibody . The EC50 is 0.3675 - 0.65842 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesFOLR

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor); Apical cell membrane (Lipid-anchor, GPI-anchor); Basolateral cell membrane (Lipid-anchor, GPI-like-anchor); Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome
Tissue Specificity: Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium
Disease Association: Neurodegeneration due to cerebral folate transport deficiency (NCFTD) : An autosomal recessive neurodegenerative disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy and leukodystrophy. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Folate receptor alpha(FOLR1),partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 25-233aa, with C-terminal 10xHis-tagged tag, molecular weight 25.9 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PTK7 at 2 μg/mL can bind Anti-PTK7 recombinant antibody . The EC50 is 0.3675 - 0.65842 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor); Apical cell membrane (Lipid-anchor, GPI-anchor); Basolateral cell membrane (Lipid-anchor, GPI-like-anchor); Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome

Disease Association

Neurodegeneration due to cerebral folate transport deficiency (NCFTD) : An autosomal recessive neurodegenerative disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy and leukodystrophy. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium

Gene: FOLR1  |  Organism: Homo sapiens  |  Synonyms: Adult folate-binding protein; Folate receptor 1; Folate receptor, adult; KB cells FBP; Ovarian tumor-associated antigen MOv18
Key Publications

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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