Recombinant Human Glucose-6-phosphate isomerase (GPI), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 66.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Glucose-6-phosphate isomerase (GPI), partial

Recombinant Human Glucose-6-phosphate isomerase (GPI), partial — Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Purity >90%.

SKU: BCRECP-000779 Categories: ,
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Product Specifications

Product SkuBCRECP-000779
Product DescriptionRecombinant Human Glucose-6-phosphate isomerase (GPI) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-554aa. [Accession | P06744].
Uniprot No.P06744
Gene NamesGPI
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-554aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight66.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6-phosphate and M6P.

Biological Context

Subcellular Location: Cytoplasm; Secreted
Disease Association: Anemia, congenital, non-spherocytic hemolytic, 4 (CNSHA4) : An autosomal recessive form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4

Product Specifications

Recombinant Human Glucose-6-phosphate isomerase (GPI), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-554aa, with N-terminal 6xHis-tagged tag, molecular weight 66.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm (PubMed:28803808). Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P) (By similarity). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6-phosphate and M6P (By similarity). In addition to its metabolic role, this enzyme functions extracellularly as a cytokine: acts as autocrine motility factor (AMF), a secreted angiogenic factor that enhances endothelial cell motility (PubMed:11437381). Functions as neuroleukin, a neurotrophic factor supporting the survival of spinal and sensory neurons (PubMed:11004567, PubMed:3352745). Released by lectin-stimulated T-cells to induce immunoglobulin secretion (PubMed:11004567, PubMed:3352745)

Subcellular Location

Cytoplasm; Secreted

Disease Association

Anemia, congenital, non-spherocytic hemolytic, 4 (CNSHA4) : An autosomal recessive form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homodimer; in the catalytically active form (PubMed:11371164, PubMed:12054796, PubMed:12777791). Monomer in the secreted form (PubMed:11371164, PubMed:12054796, PubMed:12777791)

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4

Gene: GPI  |  Organism: Homo sapiens  |  Synonyms: Autocrine motility factor; D-hexose-6-phosphate anomerase; Glucose-6-phosphate C2-epimerase; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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