Recombinant Mouse Glucose-6-phosphate isomerase (Gpi), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged, 64.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Glucose-6-phosphate isomerase (Gpi)

Recombinant Mouse Glucose-6-phosphate isomerase (Gpi) — Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Purity >90%.

SKU: BCRECP-002904 Categories: ,
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Product Specifications

Product SkuBCRECP-002904
Product DescriptionRecombinant Mouse Glucose-6-phosphate isomerase (Gpi) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 2-558aa. [Accession | P06745].
Uniprot No.P06745
Gene NamesGpi
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-558aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight64.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm. Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6-phosphate and M6P.

Biological Context

Subcellular Location: Cytoplasm; Secreted
Pathway: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4

Product Specifications

Recombinant Mouse Glucose-6-phosphate isomerase (Gpi) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 2-558aa, with N-terminal 6xHis-tagged tag, molecular weight 64.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Isomerase that catalyzes the conversion of alpha-D-glucose-6-phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm (PubMed:2344351, PubMed:7277315, PubMed:8417789). Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P) (By similarity). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6-phosphate and M6P (By similarity). In addition to its metabolic role, this enzyme functions extracellularly as a cytokine: acts as autocrine motility factor (AMF), a secreted angiogenic factor that enhances endothelial cell motility (By similarity). Functions as neuroleukin, a neurotrophic factor supporting the survival of spinal and sensory neurons (PubMed:3352745, PubMed:3764429). Released by lectin-stimulated T-cells to induce immunoglobulin secretion (PubMed:3352745)

Subcellular Location

Cytoplasm; Secreted

Subunit

Homodimer in the catalytically active form, monomer in the secreted form

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4

Gene: Gpi  |  Organism: Mus musculus  |  Synonyms: Autocrine motility factor; D-hexose-6-phosphate anomerase; Glucose-6-phosphate C2-epimerase; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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