Recombinant Human Glutathione peroxidase 1 (GPX1) (U49S), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 26.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Glutathione peroxidase 1 (GPX1) (U49S)

Recombinant Human Glutathione peroxidase 1 (GPX1) (U49S) — Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Purity >90%.

SKU: BCRECP-000782 Categories: ,
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Product Specifications

Product SkuBCRECP-000782
Product DescriptionRecombinant Human Glutathione peroxidase 1 (GPX1) (U49S) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-203aa(U49S). [Accession | P07203].
Uniprot No.P07203
Gene NamesGPX1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-203aa(U49S)
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

Biological Context

Subcellular Location: Cytoplasm; Mitochondrion
Tissue Specificity: Expressed in platelets (at protein level)

Product Specifications

Recombinant Human Glutathione peroxidase 1 (GPX1) (U49S) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-203aa(U49S), with N-terminal 6xHis-tagged tag, molecular weight 26.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402)

Subcellular Location

Cytoplasm; Mitochondrion

Tissue Specificity

Expressed in platelets (at protein level)

Subunit

Homotetramer. Interacts with MIEN1

Gene: GPX1  |  Organism: Homo sapiens  |  Synonyms: Cellular glutathione peroxidase; Phospholipid-hydroperoxide glutathione peroxidase GPX1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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