Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 15.5 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-001177 Categories: ,
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Product SKUBCACP-001177
Product DescriptionRecombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 18-144aa. [Accession | P04141].
Uniprot No.P04141
Gene NamesCSF2
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region18-144aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight15.5 kDa
ActivityThe ED50 as determined in a cell proliferation assay using PDC-P1 cells is 40-170 pg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesColony stimulating factor 2 (granulocyte-macrophage); Colony Stimulating Factor 2; Colony stimulating factor; Colony-stimulating factor; CSF 2; CSF; CSF2; CSF2_HUMAN; GM-CSF; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; Granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGI1GM; Molgramostin; Pluripoietin-a; Sargramostim

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 18-144aa, with C-terminal 6xHis-tagged tag, molecular weight 15.5 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes

Subcellular Location

Secreted

Subunit

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits

Gene: CSF2  |  Organism: Homo sapiens  |  Synonyms: Colony-stimulating factor; Molgramostin; Sargramostim
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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