Recombinant Human High mobility group protein B1 (HMGB1), Homo sapiens (Human), Yeast, N-terminal 6xHis-tagged, 26.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human High mobility group protein B1 (HMGB1)

Recombinant Human High mobility group protein B1 (HMGB1) — (Microbial infection) Facilitates dengue virus propagation via interaction with the untranslated regions of viral genome. Purity >90%.

SKU: BCRECP-000841 Category:
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Product Specifications

Product SkuBCRECP-000841
Product DescriptionRecombinant Human High mobility group protein B1 (HMGB1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 2-215aa. [Accession | P09429].
Uniprot No.P09429
Gene NamesHmgb1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-215aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight26.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

(Microbial infection) Facilitates dengue virus propagation via interaction with the untranslated regions of viral genome. In turn, this interaction with viral RNA may regulate secondary structure of dengue RNA thus facilitating its recognition by the replication complex.

Biological Context

Subcellular Location: Endoplasmic reticulum
Tissue Specificity: Ubiquitous. Expressed in platelets

Product Specifications

Recombinant Human High mobility group protein B1 (HMGB1) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 2-215aa, with N-terminal 6xHis-tagged tag, molecular weight 26.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Facilitates dengue virus propagation via interaction with the untranslated regions of viral genome. In turn, this interaction with viral RNA may regulate secondary structure of dengue RNA thus facilitating its recognition by the replication complex

Subcellular Location

Endoplasmic reticulum

Tissue Specificity

Ubiquitous. Expressed in platelets (PubMed:11154118)

Subunit

(Microbial infection) Interacts with influenza A virus protein NP; this interaction promotes viral replication

Gene: HMGB1  |  Organism: Homo sapiens  |  Synonyms: High mobility group protein 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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