Recombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1), Homo sapiens (Human), E.coli, Tag-Free, 44.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1)

Recombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-001621 Categories: ,
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Product Specifications

Product SkuBCRECP-001621
Product DescriptionRecombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1) Protein is expressed from E.coli with Tag-Free. It contains 1-397aa. [Accession | Q15047].
Uniprot No.Q15047
Gene NamesSETDB1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-397aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight44.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Lacks all domains required for histone methyltransferase activity.

Biological Context

Subcellular Location: Nucleus; Cytoplasm; Chromosome
Tissue Specificity: Widely expressed. High expression in testis

Product Specifications

Recombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-397aa, with Tag-Free tag, molecular weight 44.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Lacks all domains required for histone methyltransferase activity

Subcellular Location

Nucleus; Cytoplasm; Chromosome

Tissue Specificity

Widely expressed. High expression in testis

Subunit

Part of a complex containing at least CDYL, REST, WIZ, SETDB1, EHMT1 and EHMT2 (PubMed:19061646). Forms a complex with ATRX, TRIM28 and ZNF274 (PubMed:27029610). Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1 (PubMed:24623306). Interacts with TRIM28/TIF1B (PubMed:11959841). Interacts with ATF7IP and ATF7IP2; the interaction with ATF7IP protects SETDB1 from proteasomal degradation and is required to stimulate histone methyltransferase activity and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (PubMed:14536086, PubMed:15691849). Interacts with CBX1 and CBX5 (PubMed:15899859). Interacts with DNMT3A and DNMT3B (PubMed:16682412). Interacts with SUMO2. Interacts with MPHOSPH8 (PubMed:20871592). Interacts with ERG (By similarity). Interacts with HDAC1, HDAC2, SIN3A and SIN3B (By similarity). Interacts with ATRX. Interacts with RESF1 (By similarity). Interacts with ZNF638 (PubMed:30487602). Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression (PubMed:32051553). Interacts with PHF13; the interaction probably enhances SETDB1 chromatin-associated levels and activity (PubMed:23034801). Interacts with VRK1 (PubMed:37179361)

Gene: SETDB1  |  Organism: Homo sapiens  |  Synonyms: ERG-associated protein with SET domain; Histone H3-K9 methyltransferase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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