Recombinant Human Homeobox protein NANOG (NANOG)

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation

Subcellular Location

Nucleus

Tissue Specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes

Subunit

Interacts with SMAD1 (By similarity). Interacts with SALL4 (By similarity). Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity). Interacts with NSD2 (By similarity)

• Mitsui K. et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. — Cell (2003)
• Clark A.T. et al. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. — Stem Cells (2004)
• Kim J.S. et al. Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog. — Exp. Mol. Med. (2005)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Mural R.J. et al. PubMed: CI-5GBD0VIIJ7C63 (SEP-)
• Chambers I. et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. — Cell (2003)
• Hart A.H. et al. Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human. — Dev. Dyn. (2004)
• Oh J.-H. et al. Identification of a putative transactivation domain in human Nanog. — Exp. Mol. Med. (2005)