Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpr (vpr), Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025) (HIV-1), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 18.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpr (vpr)

Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpr (vpr) — During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. Purity >85%.

SKU: BCRECP-004741 Categories: ,
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Product Specifications

Product SkuBCRECP-004741
Product DescriptionRecombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpr (vpr) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-96aa. [Accession | O12160].
Uniprot No.O12160
Gene Namesvpr
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-96aa
SpeciesHuman immunodeficiency virus type 1 group M subtype C (isolate 92BR025) (HIV-1)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight18.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions.

Biological Context

Subcellular Location: Virion; Host nucleus; Host extracellular space

Product Specifications

Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpr (vpr) is a recombinant protein from Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025) (HIV-1), expressed in E.coli, covering amino acids 1-96aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 18.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions

Subcellular Location

Virion; Host nucleus; Host extracellular space

Subunit

Homooligomer, may form homodimer. Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C-terminus of the P6gag protein. Interacts with host UNG. May interact with host RAD23A/HHR23A. Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediating ubiquitination of host proteins such as TERT and ZGPAT and arrest of the cell cycle in G2 phase

Gene: vpr  |  Organism: Human immunodeficiency virus type 1 group M subtype C (isolate 92BR025)  |  Synonyms: R ORF protein; Viral protein R

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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